Nonsteroidal anti-inflammatory drug NS398 regulates the RECK gene expression in the prostate carcinoma strain DU145.
- Author:
Zhen-Yu XU
1
;
Jian-Ping GAO
;
Ying-Hao SUN
;
Zheng-Yu ZHANG
;
Jing-Ping GE
;
Chuan-Liang XU
;
Lin-Hui WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents, Non-Steroidal; pharmacology; Blotting, Western; Cattle; Cell Line, Tumor; GPI-Linked Proteins; Gene Expression Regulation, Neoplastic; drug effects; Humans; Male; Membrane Glycoproteins; biosynthesis; genetics; Nitrobenzenes; pharmacology; Prostatic Neoplasms; genetics; metabolism; pathology; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sulfonamides; pharmacology
- From: National Journal of Andrology 2008;14(4):303-306
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the regulative effect of the nonsteroidal anti-inflammatory drug NS398 on the RECK gene in the prostate carcinoma strain DU145.
METHODSDU145 was treated with various concentrations of NS398 for 48 hours. The mRNA level was measured by RT PCR technique and the expression of the RECK protein determined by Western blot.
RESULTSThe mRNA level of the RECK gene was obviously higher, while the MMP9 level markedly lower in the treated group than in the control, and so was the expression of the RECK protein.
CONCLUSIONNS398 induces the expression of the RECK gene, which might be the mechanism of its anti-tumor effect.
