Construction of the recombinant adenovirus vector bearing dual-survivin shRNA.
- Author:
Peng GAO
1
;
Yu-Xi SHAN
;
Dong-Rong YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Cell Line; DNA Restriction Enzymes; metabolism; Genetic Vectors; genetics; Green Fluorescent Proteins; genetics; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; genetics; Neoplasm Proteins; genetics; Polymerase Chain Reaction; RNA, Small Interfering; genetics; Recombinant Fusion Proteins; genetics; metabolism; Transfection
- From: National Journal of Andrology 2008;14(4):324-327
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a recombinant adenovirus vector bearing dual-survivin short hairpin RNA (shRNA).
METHODSDual-survivin shRNA was designed and synthesized respectively, both inserted into adenovirus DNA. The recombinant adenovirus vector was confirmed via both sequencing and restriction digestion analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenoviruses.
RESULTSThe recombinant adenovirus vector was constructed and the target sequence was obtained.
CONCLUSIONThe construction of the recombinant adenovirus vector provides a basis for the research of potential gene therapy for prostate cancer.
