An analysis on chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization.
- Author:
Lian-Bing LI
1
;
Yan-Kai XIA
;
Xin-Sheng LI
;
Jing LÜ
;
Ming-Fu MA
;
Ling SONG
;
Yuan JI
;
Ji-Gao YANG
;
Tian-Feng ZHANG
;
Xiao-Xing CHEN
;
Ying RONG
;
Le-Tian ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Aneuploidy; Asthenozoospermia; genetics; Chromosome Painting; methods; Chromosomes, Human, Pair 18; Chromosomes, Human, X; Chromosomes, Human, Y; Humans; Male; Sex Chromosome Aberrations; Spermatozoa; metabolism
- From: National Journal of Andrology 2008;14(3):211-214
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the numerical aberration of chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization.
METHODSThe experiment included 10 asthenospermia patients and 5 healthy men with normal semen quality as controls. Fluorescence in situ hybridization (FISH) and probes for chromosomes including X, Y and 18 were used to determine the frequency of the aneuploid of the chromosomes in spermatozoa.
RESULTSOf the 45,547 spermatozoa counted from the semen samples, the hybridization rate was 99.18%. The frequencies of the chromosome disomies including XX18, XY18, YY18, X1818 and Y1818 were (0.124 +/- -0.086)%, (0.360 +/- 0.380)%, (0.109 +/- 0.195)%, (0.342 +/- 0.746)% and (0.299 +/- 0.564)% in the case group and (0.014 +/- 0.019)%, (0.090 +/- 0.080)%, (0.030 +/- 0.031)%, (0.068 +/- 0.103)% and (0.075 +/- 0.083)% in the control. The sperm aneuploid rate was 9.25% in the former and 2.70% in the latter, with significant difference in between (P< 0.01).
CONCLUSIONAsthenospermia patients have a higher aneuploid rate of sperm chromosome than normal fertile men. However, larger samples are yet to be studied to obtain more scientific evidence.
