The mRNA expression of P16(ink4a) and HST2 in benign prostatic hyperplasia tissues: a pilot study.

Ying Xiong; Tie-jun Pan; Zhang-qun YE

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The mRNA expression of P16(ink4a) and HST2 in benign prostatic hyperplasia tissues: a pilot study.

Author: Ying XIONG ¹ ; Tie-Jun PAN ; Zhang-Qun YE
Author Information

1. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Publication Type:Journal Article
MeSH: Adult; Aged; Cyclin-Dependent Kinase Inhibitor p16; genetics; Fibroblast Growth Factor 6; genetics; Gene Expression Profiling; Humans; Male; Pilot Projects; Prostatic Hyperplasia; genetics; pathology; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
From: National Journal of Andrology 2008;14(3):224-226
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the expressions of the aging gene P16(ink4a) and anti-aging gene HST2 in benign prostatic hyperplasia (BPH).
METHODSTwenty-three BPH and eighteen normal prostate specimens were collected and total RNA was extracted, followed by the reverse transcriptase polymerase chain reaction (RT-PCR). The expressions of P16(ink4a) was detected by semi-quantitative analysis in BPH and normal prostate tissues.
RESULTSP16(ink4a) mRNA, rather than HST2, was expressed in the BPH and normal prostate tissues. Semi-quantitative analysis showed that the P16(ink4a) mRNA expression in the normal prostate tissues (0.4868 +/- 0.545 was significantly higher than in the BPH tissues (0.2783 +/- 0.0268, with a statistical difference in between (P < 0. 05).
CONCLUSIONP16(ink4a) might play an important role in the pathogenesis of BPH and is probably one of the factors of cell aging escape.