Calmodulin antagonists are involved in mouse sperm capacitation.
- Author:
Kun LI
1
;
Ya-Mei XUE
;
Jie-Qiang LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calmodulin; antagonists & inhibitors; Enzyme Inhibitors; pharmacology; Imidazoles; pharmacology; Male; Mice; Mice, Inbred ICR; Sperm Capacitation; drug effects; Sperm Count; Sperm Motility; drug effects; Spermatids; cytology; drug effects; physiology
- From: National Journal of Andrology 2008;14(3):234-237
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the possible involvement of calmodulin in mouse sperm capacitation.
METHODSCalmodulin antagonists W7 at the concentrations of 50, 100 and 200 micromol/L and calmidazolium (CZ) at the concentrations of 10, 20 and 30 micromol/ L, were coincubated with mouse sperm for 2 hours, respectively. The percentage of pattern B sperm was measured by chlorotetracycline staining. Then the sperm were coincubated with 100 micromol/L W7 or 10 micromol/L calmidazolium (CZ) before acrosome reaction was induced by 5 micromol/L progesterone and evaluated by the same method.
RESULTSAfter the treatment of the sperm with different concentrations of CZ or W7, the percentages of pattern B sperm decreased in a dose-dependent manner, significantly different from the control (P < 0.05). There was a statistic difference in the rate of acrosome reaction between the experiment and the control group (P < 0.01).
CONCLUSIONCalmodulin is a key protein involved in mouse sperm capacitation.
