Transforming growth factor beta upregulates the expression of invasion and metastasis associated proteins in prostate cancer LNCaP cell lines in vitro.

Author: Guang-Feng ZHU ¹ ; He-Peng CHENG ; Kai-Jie WU ; Lin-Lin ZHANG ; Guo-Dong ZHU ; Dong ZHANG ; Da-Lin HE
Author Information

1. Urology Institute of Xi'an Jiaotong University, Department of Urology, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, China. zhuguangfeng@stu.xjtu.edu.cn
Publication Type:Journal Article
MeSH: Blotting, Western; Cadherins; biosynthesis; Cell Line, Tumor; Humans; Male; Prostatic Neoplasms; metabolism; pathology; Transforming Growth Factor beta; pharmacology; Up-Regulation; drug effects; Vimentin; biosynthesis
From: National Journal of Andrology 2008;14(3):238-241
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo determine the effect of the transforming growth factor beta (TGF-beta) on the expression of invasion and metastasis associated proteins in the prostate cancer LNCaP cell line in vitro.
METHODSThe prostate cancer cell line LNCaP was treated with TGF-beta in vitro. Western blotting was used to detect the expression of the "invasion and metastasis" associated proteins E-Cadherin, N-Cadherin and Vimentin.
RESULTSThe expression of N-Cadherin and Vimentin of the LNCaP cells treated with TGF-beta for 12 hours was significantly upregulated, but not that of E-Cadherin.
CONCLUSIONTGF-beta may induce epithelial-mesenchymal transition (EMT) of LNCaP cells which might be of importance in promoting prostate cancer cells invading to ambient tissues and metastasizing to distant organs.