Analysis of the kinematic parameters of hyperactivated Guinea pig spermatozoa.
- Author:
Lu XU
1
;
Jing-Yan HUANG
;
Qun-Wei CUI
;
Gen-Lin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Guinea Pigs; Kinetics; Male; Numerical Analysis, Computer-Assisted; Sperm Capacitation; physiology; Sperm Motility; physiology; Spermatozoa; physiology
- From: National Journal of Andrology 2008;14(5):387-390
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the role of sperm kinematic parameters in the hyperactivation of Guinea pig spermatozoa, and to confirm the index of their hyperactivated motility.
METHODSComputer-aided sperm analysis (CASA) was used to describe the kinesis parameters of the Guinea pig spermatozoa incubated for 1, 3, 5 and 7 hours.
RESULTSThe curvilinear velocity, average path velocity and amplitude of lateral head displacement were increased with time and reached the peak at 5 hours, while the straight linear velocity, linearity, straightness and beat cross frequency were gradually decreased with time and hit the bottom at 5 hours.
CONCLUSIONThe sperm movement pattern changes greatly before hyperactivation during the capacitation of Guinea pig spermatozoa.