Longbixiao capsule reduces expressions of TGF-beta1 and Smoothelin in human prostatic stromal cells cultured in vitro.
- Author:
Wei-Guo MA
1
;
Jin-Ming JIA
;
Yong-Zheng JIAO
;
Jia-Hui WANG
;
Shao-Bo LUO
;
Ju ZHANG
;
Jian-Dang SHI
;
Zhi-Song ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Capsules; Cells, Cultured; Cytoskeletal Proteins; genetics; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; chemistry; pharmacology; Gene Expression; drug effects; Humans; Male; Muscle Proteins; genetics; Prostatic Hyperplasia; pathology; Rabbits; Reverse Transcriptase Polymerase Chain Reaction; Serum; chemistry; Stromal Cells; drug effects; metabolism; pathology; Transforming Growth Factor beta1; genetics
- From: National Journal of Andrology 2008;14(5):466-470
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of the Chinese herbal medicine of Longbixiao (LBX) Capsule on the expressions of TGF-beta1 and Smoothelin in human prostatic stromal cells cultured in vitro.
METHODSBlood serum medicated with LBX was incubated with the stromal cells isolated from men with benign prostatic hyperplasia (BPH) and cultured in vitro. The mRNA expression levels of TGF-beta1 and Smoothelin were detected by real-time RT-PCR and other relevant techniques.
RESULTSIn the high and low concentration groups, the gene relative expressions of TGF-beta1 were (0.158 +/- 0.020) and (0.169 +/- 0.020) , while those of Smoothelin were (0.035 +/- 0.007) and (0.036 +/- 0.007) respectively, both significantly decreased in comparison with the control group(P < 0.01).
CONCLUSIONLBX reduces the mRNA expressions of TGF-beta1 and Smoothelin in human prostatic stromal cells and can be used in the treatment of BPH.
