17 beta-estradiol inhibits T-type calcium channels in mouse spermatogenic cells.
- Author:
Liang LU
1
;
Chang-song WANG
;
Xiao-hua GAO
;
Jun WANG
;
Jie CHENG
;
Rong GAO
;
Hang XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium Channels, T-Type; physiology; Cells, Cultured; Dose-Response Relationship, Drug; Estradiol; pharmacology; Male; Membrane Potentials; drug effects; Mice; Patch-Clamp Techniques; Spermatids; cytology; drug effects; physiology
- From: National Journal of Andrology 2008;14(6):483-488
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of 17 beta-estradiol (E2) on T-type calcium currents (ICaT) in spermatogenic cells.
METHODSCa2+ currents were obtained in acutely dissociated mouse spermatogenic cells by the whole-cell patch clamp technique and the effects of E2 on ICaT were observed.
RESULTSE2 at the concentrations of 1, 10 and 100 micromol/L significantly inhibited ICaT in the mouse spermatogenic cells, with the KC50 value of 8.89 micromol/L and the inhibition rates of (13.48 +/- 1.86) %, (28.98 +/- 2.70) and (52.93 +/- 3.42)%, respectively (n = 6, P < 0.05). E2 of 100 micromol/L significantly changed the activation and inactivation of ICaT: the half activation potential (Va 1/2) and the activation steepness factor (Ka) from ( -48.94 +/- 0.22) mV and (5.19 +/- 0.19) mV to (-54.34 +/- 1.02) mV and (6.02 +/- 0.84) mV ( n = 5, P < 0.05) , and the half inactivation potential (Vi 1/2) and the inactivation steepness factor (Ki) from (-56.51 +/- 0.13) mV and (3.36 +/- 0.11) mV to (-61.78 +/- 0.50) mV and (4.25 +/- 0.37) mV, respectively (n = 5, P < 0.05).
CONCLUSIONE2 has a significant inhibitory effect on ICaT in mouse spermatogenic cells in a con-centration-dependent manner.
