Effects of testosterone on the proliferation of rat corpus cavernosum cells in vitro.
- Author:
Bo LIU
1
;
Ji-hong LIU
;
Tao WANG
;
Jun YANG
;
Shao-gang WANG
;
Ru-zhu LAN
;
Zhang-qun YE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Fibroblasts; cytology; drug effects; metabolism; Immunohistochemistry; Male; Myocytes, Smooth Muscle; cytology; drug effects; metabolism; Penis; cytology; metabolism; Rats; Rats, Sprague-Dawley; Receptors, Androgen; metabolism; Testosterone; pharmacology
- From: National Journal of Andrology 2008;14(6):524-526
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of testosterone on the proliferation of penile corpus cavernosal cells in male SD rats.
METHODSSmooth muscle cells (SMCs) and fibroblasts collected from the corpus cavernosal tissues of male SD rats were cultured by the enzymatic dispersion method and detected for the expression of the androgen receptor (AR) by immunohistochemistry. The effects of testosterone on the SMCs and fibroblasts were observed by methyl thiazolyl tetrazolium (MTT) assay in different concentration groups (10(-8) mol/L, 10(-7) mol/L, 10(-6) mol/L, 10(-5) mol/L, 10(-4) mol/L and 10(-3) mol/L) in comparison with the control.
RESULTSThe AR expression was found in the penile corpus cavernosal tissues. MTT assay showed that, at the concentration of 10(-5) mol/L, testosterone induced the proliferation of SMCs (68100 +/- 2200) and fibroblasts (70200 +/- 1300), with significant differences from the control ( P < 0.05), while at 10(-4) mol/L, it inhibited their proliferation (55000 +/- 1400 and 59100 +/- 1500, respectively), (P < 0.01). No significant effects were noted in the other concentration groups.
CONCLUSIONAR exists in the penile corpus cavernosal tissues of male rats. Testosterone modulates the proliferation of corpus cavernosum tissue cells through AR, and different concentrations of testosterone may be positively or negatively correlated with the proliferation of SMCs and fibroblasts.
