Expression of constructed eukaryotic vectors carrying encoding gene of soluble human interleukin-1 receptor in mammalian cells.
- Author:
Yan XU
1
;
Peng LI
;
Yunhui ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CHO Cells; COS Cells; Cercopithecus aethiops; Cloning, Molecular; Cricetinae; Cricetulus; DNA, Complementary; biosynthesis; genetics; Enzyme-Linked Immunosorbent Assay; Eukaryotic Cells; metabolism; Genetic Vectors; Humans; Plasmids; genetics; Receptors, Interleukin-1; biosynthesis; genetics; Recombinant Fusion Proteins; biosynthesis; genetics; Recombination, Genetic; Transcription, Genetic; Transfection
- From: West China Journal of Stomatology 2003;21(6):467-470
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study is to make sure if the constructed pcDNA3 carrying encoding gene of sIL-1R can be expressed in mammalian cells in vitro.
METHODSCOS-1 cells and CHO cells were respectively transfected with recombinant plasmid pcDNA3/sIL-1R by liposome. The protein expression products were detected by ELISA.
RESULTSThe results indicated that the protein expression products could be detected in the cell plasma and the cell culture supermatant. The expression level in experimental groups was much higher than that in control groups(P < 0.05).
CONCLUSIONThe constructed pcDNA3/sIL-1R can express interest protein in mammalian cells and this establishes the basis for future investigation on gene therapy of periodonititis and other inflammatory diseases.
