Study on tumor necrosis factor-alpha and interleukin 2 in rat skin allograft.

Author: Junlin WANG ¹ ; Yan JIN ; Xiaoliang LIU
Author Information

1. Department of Oral Histology and Pathology, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Publication Type:Journal Article
MeSH: Animals; Animals, Newborn; Cells, Cultured; Graft Rejection; immunology; Interleukin-2; pharmacology; Male; Rats; Rats, Sprague-Dawley; Rats, Wistar; Skin; cytology; immunology; Skin Transplantation; Tissue Engineering; Tumor Necrosis Factor-alpha; pharmacology
From: West China Journal of Stomatology 2003;21(6):471-480
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo elucidate the histocompatibility of tissue engineered rat skin through studying the effect of TNF-alpha and IL-2 in immunological rejection after rat skin allograft.
METHODSTissue engineered skin that the basic materials were taken from neonatal SD rats was cultured in lab, grafted to adult Wistar rats. The expressions of TNF-alpha and IL-2 in grafted tissue were detected with immunohistochemical staining and Western blot.
RESULTSThe expressions of TNF-alpha and IL-2 were remarkable in skin allograft group, but low in tissue engineered skin group.
CONCLUSIONThe expressions of TNF-alpha and IL-2 and the immunological rejection were closely related after skin allograft, but the tissue engineered skin has favorable histocompatibility and doesn't arose obvious immunological rejections.