Expression of GBD gene of Streptococcus mutans glucan binding protein A in mammalian cells.
- Author:
Ling-yun SU
1
;
Bu-ling WU
;
Fu-yang LI
;
Qun LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Surface; biosynthesis; genetics; immunology; Bacterial Proteins; biosynthesis; genetics; immunology; COS Cells; Carrier Proteins; biosynthesis; genetics; immunology; Dental Caries; prevention & control; Eukaryotic Cells; metabolism; Gene Expression; Genetic Vectors; Humans; Lectins; Mammals; Plasmids; genetics; immunology; Recombinant Proteins; Streptococcus mutans; genetics; metabolism; Transfection; Vaccines, DNA
- From: West China Journal of Stomatology 2004;22(1):10-12
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the expression of recombinant plasmid pcDNA3.1/GBD of glucan binding protein of Streptococcus mutans in mammalian cells COS-7.
METHODSEukaryotic plasmid carrying encoding gene of GBD of Streptococcus mutans gbpA was constructed and the plasmid was introduced into COS-7 cells by Lipofectamine reagent. The transient expressed protein in COS-7 cells was detected by immunochemistry technique.
RESULTSThe positive expression was detected in plasma of the cells which were transfected with recombinant plasmid pcDNA3.1/GBD. The cells which were transfected with pcDNA3.1 were negative.
CONCLUSIONGBD can translate and express in COS-7 cells after transfected with recombinant plasmid pcDNA3.1/GBD. The expressed protein locates in the plasma and the protein is able to combine with anti-GbpA antibody. The expressed protein has the antigenicity and is a candidate gene vaccine.
