Expression of matrixmetalloproteinase-8 on the bell-stage in human and rat tooth development.
- Author:
Yu-qing HAO
1
;
Zhong-ying NIU
;
Gou-quan WANG
;
Xue-dong ZHOU
;
Tao HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Dentin; enzymology; Embryo, Mammalian; In Situ Hybridization; Matrix Metalloproteinase 8; biosynthesis; genetics; Maxilla; enzymology; Odontogenesis; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Sprague-Dawley; Tooth Germ; embryology; enzymology
- From: West China Journal of Stomatology 2004;22(1):26-28
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of MMP-8 in human and rat tooth development.
METHODSImmunohistochemistry was used to detect the localization of MMP-8 protein while in situ hybridization was used to examine the expression of MMP-8 mRNA.
RESULTSThe expression of MMP-8 protein was localized in odontoblast and dentin matrix at the later bell stage in human tooth germ. The dentin was denser close to the pulp cavity. The expression of MMP-8 mRNA was found in very few polarized odontoblast at the early bell stage and all polarized odontoblast at the later bell stage in rat tooth germ.
CONCLUSIONThe results suggested that MMP-8 involved in dentin matrix rebuilding in the process of dentin formation in human and rat dental development.
