Characterization of Notch gene involved in genetic regulatory networks in dental pulp stem cells.
- Author:
Qun LU
1
;
Bu-ling WU
;
Ji-shu WANG
;
Hua HAN
;
Xue-dong ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Cells, Cultured; Dental Pulp; cytology; metabolism; Membrane Proteins; biosynthesis; genetics; Mice; Mice, Inbred BALB C; RNA, Messenger; biosynthesis; genetics; Receptors, Cell Surface; biosynthesis; genetics; Receptors, Notch; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Stem Cells; metabolism
- From: West China Journal of Stomatology 2004;22(1):54-56
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the characterization of Notch gene involved in genetic regulatory networks in dental pulp stem cells.
METHODSThe pulp tissue was separated from mouse teeth and digested by collagenase type I. Single-cell suspensions of dental pulp were seeded into 6-well plates with alpha modification of Eagle's medium supplemented with ES cell qualified Fetal Bovine Serum. Colony-forming efficiency was assessed in 14ds culture. Transcripts for Notch were detected by reverse transcription-PCR by using total RNA isolated from cells.
RESULTSThere were clonogenic cells in dental pulp cell and the incidence of colony-forming cells derived from mouse dental pulp cells was 1.6-2.5 colonies/10(4) plate. Mouse-specific Notch mRNA expressed in colony-forming cells.
CONCLUSIONNotch mRNA expressing in colony-forming cells provided a more detailed understanding of mouse dental pulp stem cell biology.
