The effect of bFGF on proliferation of periodontal fibroblast-like cells of dogs.
- Author:
Pi-shan YANG
1
;
Qin-feng SUN
;
Ai-mei SONG
;
Shao-hua GE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Division; drug effects; Dogs; Fibroblast Growth Factor 2; pharmacology; Fibroblasts; cytology; Periodontium; cytology; surgery; Regeneration; Wound Healing; drug effects
- From: West China Journal of Stomatology 2004;22(1):59-61
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effects of basic fibroblast growth factor(bFGF) on proliferation of periodontal fibroblast-like cells in vivo.
METHODSA U-shaped osseous defect was produced on the buccal side of the mesial root. Four posterior teeth were conducted in four quadrants. Each quadrant included 4 groups: control, bFGF, expanded polytetrafluoroethylene(ePTFE) membrane, bFGF + ePTFE. Each time the 4 teeth sites in one quadrant were operated weekly and each dog experienced 4 times of operations. Bromodeoxyuridine(BrdU) was injected 1 hour prior to sacrificing the dogs at 4 weeks after first surgery. Immunohistochemical method was applied to count the BrdU-labeled fibroblast-like cells.
RESULTSThe number of BrdU-labeled cells reached the maximum at the 2nd week among all groups and then, decreased with time. Both bFGF and bFGF + ePTFE treated group had significantly more BrdU+ cells than remained control or ePTFE groups (P < 0.05) at 1st, 2nd weeks after surgery.
CONCLUSION2 weeks after periodontal surgery is active phase of proliferation of periodontal fibroblasts. bFGF enhances fibroblast proliferation in early periodontal wound healing, and in turn accelerate periodontal regeneration.
