Transfection of human endostatin gene with lipofectamin and the expression of hES protein in Tca8113 cell.
- Author:
Chao-bin PAN
1
;
Hong-zhang HUANG
;
Jian-guang WANG
;
Jin-song HOU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; genetics; metabolism; pathology; Cell Division; Cloning, Molecular; Endostatins; biosynthesis; genetics; Humans; Lipids; pharmacology; Tongue Neoplasms; genetics; metabolism; pathology; Transfection; Tumor Cells, Cultured
- From: West China Journal of Stomatology 2004;22(2):96-99
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this study was to establish transfergeneic Tca8113 cell and evaluate the expression of human endostatin (hES) gene in the cell colone in vitro.
METHODSTo transfect hES gene into Tca8113 cells, lipofectamin was complexed with plasmid encoding hES gene, and blasticidin S antibiotic was adopted to select Tca8113--hES cell clone. Immunohistochemistry S-P method was adopted to detect the expression of hES in the transfergenic Tca8113 cell in vitro.
RESULTSTransfected by hES, the transfergenic Tca8113 cells could grow and proliferate in RPMI--1640 culture medium containing blasticidin S antibiotic. The expression rate of hES reached 100%.
CONCLUSIONhES gene can express in hES-transfected Tca8113 cell in vitro.
