In vitro and in vivo study on proliferation of adnoid cystic carcinoma cell lines after nm23-h1. Introduction.
- Author:
Wen LI
1
;
Yu-ming WEN
;
Li-hong WANG
;
Zhan YANG
;
Rui-lin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies; analysis; Carcinoma, Adenoid Cystic; genetics; pathology; Cell Division; Cell Line, Tumor; Female; Genetic Therapy; Ki-67 Antigen; immunology; Mice; Mice, Inbred BALB C; Mice, Nude; Mouth Neoplasms; genetics; pathology; NM23 Nucleoside Diphosphate Kinases; Neoplasm Transplantation; Nucleoside-Diphosphate Kinase; Proteins; genetics; Transfection
- From: West China Journal of Stomatology 2004;22(2):109-111
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of nm23-h1 transfection on proliferation characteristics of adnoid carcinoma cell lines in vitro and in vivo.
METHODSIn vitro ACC-M cell lines were incubated after putative anti-metastatic gene nm23-h1 was introduced into the cells with the help of G418 selective incubation base. The ACC-M cells were transplanted into 10 BALB/C nude mices subcutaneously and non-transfected cell lines were taken as control. Immunohisto chemistry and Ki67 antibody were employed to study the proliferation character of cell crawling pieces and paraffin-bedded slice, meanwhile, the solid tumor of both groups were prepared for flow cytometry(FCM).
RESULTSTransfected cells grew slower than non-transfected cells and this trend became more obvious as passages passed on. In vitro the expression of Ki67 of transfected cells was little stronger than non-transfected cells, while the expression of Ki67 in solid slices was almost negative in both groups. Transfected cells presented slower growth than non-transfected cells in the early stage (2 weeks) and 2 weeks later there was no obvious difference in size(P > 0.05). FCM value accorded well with the result.
CONCLUSIONIntroduction of nm23-h1 into the ACC-M cell lines may have transient inhibitory effects on its proliferation.
