Effect of phosphorothioate antisense Bcl-xL oligodeoxynucleotides on apoptosis and thermosensitivity of BcaCD885 cells.
- Author:
Yong-wen HE
1
;
Li BIAN
;
Xin-hua LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Flow Cytometry; Gene Expression Regulation, Neoplastic; drug effects; genetics; Hot Temperature; Humans; Hyperthermia, Induced; Mouth Neoplasms; genetics; pathology; Oligodeoxyribonucleotides, Antisense; pharmacology; Oligonucleotides, Antisense; genetics; pharmacology; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; Thionucleotides; pharmacology; Transfection; Tumor Cells, Cultured; bcl-X Protein
- From: West China Journal of Stomatology 2004;22(2):112-114
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of phosphorothioate antisense Bcl-xL oligodeoxynucleotides on apoptosis and thermosensitivity of BcaCD885 cells.
METHODSAfter phosphorothioate antisense Bcl-xL oligodeoxynucleotides were transfected into BcaCD885 cells. The characteristics of apoptotic cells were evaluated by morphological observation and TUNEL staining. Apoptotic rate and Bcl-xL protein expression were analyzed with flow cytometry. The influence of phosphorothioate antisense Bcl-xL oligodeoxynucleotides on apoptotic rate of BcaCD885 cells induced by hyperthermia with 43 degrees C 40 min was also examined through flow cytometry.
RESULTSThe BcaCD885 cells transfected with phosphorothioate antisense Bcl-xL oligodeoxynucleotides displayed apoptotic morphological features. The Bcl-xL protein expression level of these cells was down-regulated significantly compared with the controlled group (P < 0.05). The apoptotic rate of these cells induced by hyperthermia was increased significantly compared with the controlled group (P < 0.05).
CONCLUSIONPhosphorothioate antisense Bcl-xL oligodeoxynucleotides can induce apoptosis and improve thermosensitivity of BcaCD885 cells.
