Establishment of a culture system of chick embryo for mouse tooth germ development.

Author: Zheng-bin YAN ¹ ; Wei-dong TIAN ; Lei LIU ; Jin-qiu HOU ; Xi-zhe CHEN ; Zhi-yong LI
Author Information

1. Key. Laboratory of Oral Biomedical Engineering Ministry of Education, Sichuan University, Chengdu 610041, China.
Publication Type:Journal Article
MeSH: Ameloblasts; Animals; Chick Embryo; Mice; Molar; embryology; Odontoblasts; Tooth Germ; embryology
From: West China Journal of Stomatology 2004;22(3):232-234
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo establish a new culture system for mouse tooth germs in chick embryo.
METHODSThe mandibular first molar germ fragments of 15 embryonic days' Kunming mouse embryo were implanted into the lateral mesenchyme of 4-5 days' chick embryo wing buds in ove. Eggs were reincubated and implanted tissues were examined by histochemistry.
RESULTSThe cultured tooth germ development continued from cap stage to latest bell stage. The ameloblast and the odontoblast all differentiated maturely and secreted matrix.
CONCLUSION4-5 days' wing buds chick embryo could serve as developing the mouse tooth germs and demonstrate well physiological process of differentiation and morphogenesis.