Investigation of the expression of calcitonin receptor mRNA in human osteoclasts on deciduous teeth.
- Author:
Ming CHEN
1
;
Xing LIANG
;
Xiang-jun BAO
;
Hang WANG
;
Hui-qiang SUN
;
Shao-heng LU
Author Information
- Publication Type:Journal Article
- MeSH: Humans; In Situ Hybridization; In Vitro Techniques; Osteoclasts; metabolism; RNA, Messenger; biosynthesis; Receptors, Calcitonin; biosynthesis; genetics; Tooth, Deciduous; cytology; metabolism
- From: West China Journal of Stomatology 2004;22(3):235-237
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of calcitonin receptor mRNA in the osteoclasts of the resorbing deciduous teeth.
METHODSAfter fixing the collected deciduous teeth, toluidine blue was performed and tartrate-resistant acid phosphatase (TRAP) staining was used to identify the osteoclasts on the resorbing surface of human deciduous teeth and in situ hybridization of calcitonin receptor mRNA to show its existence.
RESULTSThere were a number of TRAP positive osteoclasts on the root surface which showed the expression of calcitonin receptor mRNA.
CONCLUSIONOn the resorbing surface of human deciduous teeth there are osteoclasts that express calcitonin receptor mRNA, so it is feasible to use this kind of osteoclast to test the effect of external factors on the expression of CTR mRNA.
