Interaction between osteoblasts and fibroblasts derived from same individual.
- Author:
Ping GONG
1
;
Zhen TAN
;
Qing ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Cell Culture Techniques; Cell Differentiation; Coculture Techniques; Collagen Type I; metabolism; Fibroblasts; cytology; Humans; Osteoblasts; cytology; Osteogenesis; Periodontal Ligament; cytology
- From: West China Journal of Stomatology 2004;22(4):275-277
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to determine the effect of factors pertaining to the development and metabolism of periodontal ligament, the author investigated the interaction between osteoblasts and fibroblasts.
METHODSThe cell co-culture model was established by cell culture inserts. The expression difference of protein related to mineralization (ALP, Type I collagen) between these two kinds of cells cultured individually and co-cultured were evaluated.
RESULTSCo-culture changed the ALPase activity of the two kinds of cells greatly .The ALP expression of osteoblasts decreased, but the ALP expression of periodontal ligament fibroblasts increased. The Col-I expression of these two kinds of cells did not change significantly when they were co-cultured.
CONCLUSIONPeriodontal ligament fibroblast could restrain the osteogenesis function of osteoblast, while osteoblast could induce the differentiation of fibroblast when both of them existed in periodontal tissue.
