Changes of sarco(endo)plasmic reticulum Ca2+-Mg2+-ATPase activity of cultured myoblast induced by cyclic stretch.
- Author:
Xiao-xia CHE
1
;
Hong ZENG
;
Song-jiao LUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Ca(2+) Mg(2+)-ATPase; metabolism; Cells, Cultured; Gene Expression Regulation; Male; Myoblasts; enzymology; Rats; Rats, Sprague-Dawley; Sarcoplasmic Reticulum; enzymology
- From: West China Journal of Stomatology 2004;22(4):281-286
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of stretch on sarco(endo)plasmic reticulum Ca2+-Mg2+ ATPases activity and mRNA level and study the remodeling reaction of muscle in a variety of mechanical environments.
METHODSMyoblast from maxillofacial skeletal muscle of one-week-old male Sprague-Dawley rat was cultured and stretched cyclicly using a four-point bend device. Inorganic Phosphorus test was used to compare the activity of Ca2+-Mg2+ ATPases of myoblast before and after stretch. RT-PCR was also used to observe the Ca2+-Mg2+ ATPases mRNA level.
RESULTSThe activity of Ca2+-Mg2+ ATPases of myoblast down regulated significantly in 4 hours. During the period of 8 hours to 24 hours, up-regulation followed then returned to control level at the 48 hour point. RT-PCR showed that Ca2+-Mg2+ ATPases mRNA level were elevated by stretch, particularly at 2 hour and 48 hour point.
CONCLUSIONThe results suggested a transcriptional control of Ca2+-Mg2+ ATPases activity was involved in the muscle remodeling process induced by stretch.
