Changes in adrenomedullin and receptor activity-modifying protein 2 mRNA in myocardium and vessels during L-NNA-induced hypertension in rats.
- Author:
Yong-Fen QI
1
;
Yan-Rong SHI
;
Ding-Fang BU
;
Hong-Feng JIANG
;
Lin GAO
;
Yong-Zheng PANG
;
Chao-Shu TANG
Author Information
1. Institute of Cardiovascular Research, the First Hospital, Peking University, Beijing 100034.
- Publication Type:Journal Article
- MeSH:
Adrenomedullin;
metabolism;
Animals;
Cardiomegaly;
chemically induced;
metabolism;
Hypertension;
chemically induced;
metabolism;
Myocardium;
metabolism;
Nitroarginine;
pharmacology;
RNA, Messenger;
Rats;
Receptor Activity-Modifying Protein 2;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Up-Regulation
- From:
Acta Physiologica Sinica
2002;54(4):337-341
- CountryChina
- Language:Chinese
-
Abstract:
To explore the changes in adrenomedullin (ADM) and receptor activity-modifying protein 2 (RAMP2) mRNA in myocardium and vessels in hypertension, a hypertensive rat model was prepared by administering L-NNA. Contents of ADM in plasma, myocardium and vessels were measured by radioimmunoassay (RIA). The levels of pro-ADM mRNA of myocardium and vessels were determined by competitive quantitative RT-PCR. The results showed that L-NNA induced hypertension and cardiomegaly. The ratio of heart to body weight increased by 35.5% (P<0.01). In hypertensive rats the ir-ADM in plasma, myocardium and vessels was increased by 80%, 72% and 57% (P<0.01), respectively compared with the control. The amounts of ADM mRNA in myocardium and vessels were increased by 50% and 109.2% (P<0.05), respectively, and the amounts of RAMP2 mRNA was increased by 132% and 87% (P<0.01), respectively, compared with control. The levels of ADM in myocardium and vessels were positively correlated with RAMP2 mRNA, the correlation coefficients were 0.741 and 0.885 (P<0.01), respectively. The results obtained indicate that in hypertensive rats, ADM is elevated in plasma, myocardium and ves-myocardium and vessel, and ADM and RAMP2 mRNA are up-regulated in myocardium and vessel. The ADM/RAMP2 system may play an important role in the pathogenesis of hypertension.
