Effects of low concentration of dihydroouabain on intracellular calcium in guinea pig ventricular myocytes.
- Author:
Jing-Xiang YIN
1
;
Yong-Li WANG
;
Qing LI
;
Zhong-Lin SHANG
;
Su-Wen SU
Author Information
1. Department of Pharmacology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Calcium;
metabolism;
Guinea Pigs;
Heart Ventricles;
cytology;
Male;
Myocytes, Cardiac;
cytology;
drug effects;
metabolism;
Ouabain;
analogs & derivatives;
pharmacology;
Patch-Clamp Techniques
- From:
Acta Physiologica Sinica
2002;54(5):385-389
- CountryChina
- Language:English
-
Abstract:
The effects of low concentration of dihydroouabain (DHO) on intracellular calcium concentration ([Ca(2+)](i)) were investigated in guinea pig ventricular myocytes. [Ca(2+)](i) was detected by confocal microscopy and represented by fluorescent intensity. DHO (1 fmol/L~1 mmol/L) increased [Ca(2+)](i), especially at 10 pmol/L. Nisoldipine, egtazic acid, or tetrodotoxin partially inhibited the effect of 10 pmol/L DHO on [Ca(2+)](i). The effects of DHO remained in the absence of extracellular K(+) and Na(+). These results suggest that low concentration of DHO might increase [Ca(2+)](i) via the receptor-operated Ca(2+) channels, TTX-sensitive Na(+) channels or/and triggering of intracellular calcium release; Na(+)/K(+) pump and Na(+)/Ca(2+) exchange seem not involved in the effect of DHO.
