Effect of a proteinase-activated receptor-2 (PAR-2) agonist on tryptase release from human mast cells.
- Author:
Shao-Heng HE
1
;
Hua XIE
;
Yong-Song HE
Author Information
1. Allergy and Inflammation Research Institute, the Medical College of Shantou University, Shantou 515031. shaohenghe@hotmail.com
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Humans;
Mast Cells;
drug effects;
metabolism;
Receptor, PAR-2;
agonists;
Tryptases;
metabolism
- From:
Acta Physiologica Sinica
2002;54(6):531-534
- CountryChina
- Language:Chinese
-
Abstract:
Proteinase-activated receptor-2 (PAR-2) expression has been observed on numerous cell types. However, little is known about the functional expression of PAR-2 in human mast cells. In the current study, the actions of a PAR-2 agonist trans-cinnamoyl-Leu-Ile-Gly-Arg-Leu-Orn-amide (tc-LIGRLO) on tryptase release from dispersed human colonic mast cells were examined. The results showed that tc-LIGRLO was able to induce a fold increase in tryptase release over the basal level following a 15 min incubation of colonic mast cells, whereas tc-OLRGIL did not have any effect on tryptase release. The potency of tc-LIGRLO appeared greater than that of anti-IgE and calcium ionophore A23187 (CI) in induction of tryptase release. Extending the incubation time to 30 min had no significant effect on the actions of tc-LIGRLO or anti-IgE. In the time course study, it was observed that the tryptase release from mast cells induced by tc-LIGRLO started at 1 min and peaked at 3 min following incubation. The above-mentioned results indicate that tc-LIGRLO is a potent stimulus of tryptase release from human mast cells, which strongly suggests that PAR-2s are expressed in human mast cells.
