Expression of adrenomedullin and its receptor mRNA in the tissues of normal adrenal medulla and pheochromocytoma.
- Author:
Dong-Mei LIU
1
;
Zheng-Pei ZENG
;
Han-Zhong LI
;
Xin-Rong FAN
;
Guo-Qiang LIU
;
Wei-Gang YAN
;
An-Li TONG
;
Xin ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adrenal Gland Neoplasms; metabolism; Adrenal Medulla; metabolism; Adrenomedullin; Adult; Calcitonin Gene-Related Peptide; biosynthesis; genetics; Female; Humans; Intracellular Signaling Peptides and Proteins; genetics; Male; Membrane Proteins; biosynthesis; genetics; Middle Aged; Peptides; genetics; metabolism; Pheochromocytoma; metabolism; RNA, Messenger; biosynthesis; genetics; Receptor Activity-Modifying Protein 2; Receptor Activity-Modifying Proteins; Receptors, Adrenomedullin; Receptors, Calcitonin; biosynthesis; genetics; Receptors, Peptide; metabolism
- From: Acta Academiae Medicinae Sinicae 2005;27(4):452-456
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of human adrenomedullin (ADM) and its receptor-receptor activity modifying protein 2/calcitonin receptor-like receptor (RAMP2/CRLR) mRNA in the tissues of normal adrenal medulla and pheochromocytoma.
METHODSTotal RNA was extracted from normal adrenal medulla and pheochromocytomas. The expression of ADM and RAMP2/CRLR mRNA were studied by reverse transcription-polymerase chain reaction. The ratios of ADM/GAPDH, RAMP2/ GAPDH, CRLR/GAPDH were used to evaluate the expression levels of ADM, RAMP2 and CRLR mRNA.
RESULTSExpressions of ADM and its receptor- RAMP2/CRLR mRNA were detected in normal adrenal medulla and pheochromocytoma tissues. ADM/GAPDH were 0.48+/-0.09 and 0.75+/-0.24, RAMP2/ GAPDH 0.79+/-0.12 and 1.29+/-0.30, CRLR/GAPDH 0.40+/-0.08 and 0.87+/-0.22 in normal adrenal medulla and pheochromocytomas, respectively (P < 0.05).
CONCLUSIONADM exerts a possible autocrine or paracrine effect in the adrenal. ADM may be involved in the pathogenesis of pheochromocytoma.
