Effect of different culture media on viability and tau protein expression in rat hippocampal slices.
- Author:
Ying-Chun ZHANG
1
;
Xiao-Mei LIAO
;
Jian-Zhi WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Culture Media; Hippocampus; growth & development; metabolism; L-Lactate Dehydrogenase; biosynthesis; Organ Culture Techniques; methods; Rats; Rats, Wistar; tau Proteins; biosynthesis; genetics
- From: Acta Academiae Medicinae Sinicae 2005;27(4):513-517
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of different culture media on viability and expression of tau protein in organotypic hippocampal slice.
METHODSBrain slices (400 microm) from 1, 2, 4, and 8 week-old Wistar rats were prepared and cultured in minimum essential medium (MEM) or Dulbecco's modified eagle medium: nutrient mixture (DMEM/F12) medium respectively for 21 days. Viability of the slices was measured by lactate dehydrogenase (LDH) assay and expression of tau protein was detected by Western blot.
RESULTSThe viability of the slices was not influenced significantly by the two different culture media, while the expression level of tau protein was significantly higher in DMEM/F12 than in MEM (P < 0.05), especially in the slices from 2 and 4 week-old rats.
CONCLUSIONThe slices from 2 or 4 week-old rat hippocampi and DMEM/F12 medium may be the preferred choice for tau associated researches. An ideal Alzheimer's disease model may be established based on the results of these researches.
