Establishment, characterization, and biological analysis of pancreatic adenocarcinoma cell strain SW1990/FU.
- Author:
Jun-chao GUO
1
;
Yu-pei ZHAO
;
Quan LIAO
;
Ge CHEN
;
Li-yang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; drug therapy; pathology; Animals; Antimetabolites, Antineoplastic; pharmacology; Cell Cycle; drug effects; Cell Line, Tumor; Drug Resistance, Neoplasm; genetics; Fluorouracil; pharmacology; Humans; Mice; Mice, Nude; Pancreatic Neoplasms; drug therapy; pathology
- From: Acta Academiae Medicinae Sinicae 2005;27(5):592-596
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a 5-fluorouracil (5-FU)-resistant pancreatic adenocarcinoma (PAC) cell strain, and to investigate its biological characteristics.
METHODSThe PAC cell strain SW1990 was selected into a multidrug-resistant cell strain stepwise with 5-FU, one of the most common drugs used in PAC chemotherapy, for 12 months and subsequently named SW1990/FU. The cell strain was characterized in terms of morphology, biology, and cross-resistance to adriamycin(ADM), mitomycin-C (MMC), and gemcitabine. BHLB/c-m nude mice tumor growth and CEA and CA19-9 levels were analyzed. In addition, karyotyping and FACS analysis were performed in SW1990/FU and SW1990.
RESULTSThe SW1990/FU cell strain was 132.7 times more resistant to 5-FU than the parental SW1990 cells, and exhibited cross-resistance to other agents. Compared to the parental cells, SW1990/FU cells exhibited a smaller growth rate, delayed cell-doubling time, and specific changes in chromosomes 18. Tumor diameters in multidrug resistance and parental cells inoculated in in vivo experiments were (1.5 +/- 0.30) cm and (0.8 +/- 0.15) cm, respectively.
CONCLUSIONSMorphological adaptation and intracellular changes can be induced by drug challenge in PAC cells. SW1990/FU may be used as an experimental system for the search to overcome drug resistance and to elucidate possible mechanisms of acquired drug resistance in human pancreatic cancer.