Metallothionein and its isoform genes expression in the human pancreatic cancer cell strains and their function.
- Author:
Yong XIE
1
;
Yu-pei ZHAO
;
Ge CHEN
;
Chun-hui YUAN
;
Li-jun LI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Division; genetics; Drug Resistance, Neoplasm; genetics; Humans; Metallothionein; biosynthesis; genetics; Pancreatic Neoplasms; genetics; metabolism; pathology; RNA, Messenger; genetics; Tumor Cells, Cultured
- From: Acta Academiae Medicinae Sinicae 2005;27(5):619-623
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the expression of metallothionein (MT) genes and proteins in six human pancreatic cancer cell strains and two human pancreatic cancer drug-resistant cell strains and to explore the relationship between the expression of the MT and pancreatic cancer cell chemo-resistance.
METHODSReverse transcriptase-polymerase chain reaction (RT-PCR) was used to determine the MT isoform-specific mRNA, and cadmium/hemoglobin saturation-electrochemistry to determine MT protein levels.
RESULTSMT protein expression in the pancreatic cancer cell strains was encoded by MT-1A, MT-1B, MT-1E, MT-1F, MT-1G, MT-1X, and MT-2A genes. The expression of MT proteins was upregulated and MT-1B, MT-1E, MT-1X, MT-2A genes overexpressed in human pancreatic cancer drug-resistant cell lines (P < 0.05).
CONCLUSIONExpressions of MT proteins and genes correlate with the proliferation and chemoresistance of human pancreatic cancer cell strains.
