Effects of Ras antisense oligoribonucleotide on multidrug resistance of pancreatic carcinoma Pc-2 cells.
- Author:
Xi CHEN
1
;
Zhao-yin QIN
;
Zhi-peng HU
;
Tao WU
;
Zong-zheng JI
;
Xin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; biosynthesis; Cell Line, Tumor; Down-Regulation; Doxorubicin; metabolism; Drug Resistance, Multiple; drug effects; genetics; Drug Resistance, Neoplasm; drug effects; genetics; Genes, MDR; drug effects; genetics; Humans; Oligonucleotides, Antisense; pharmacology; Pancreatic Neoplasms; genetics; metabolism; pathology; ras Proteins; biosynthesis; genetics
- From: Acta Academiae Medicinae Sinicae 2005;27(5):633-636
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of Ras antisense oligoribonucleotide (ASODN) in multidrug resistance (MDR) of pancreatic carcinoma Pc-2 cells.
METHODSRas and P-gp expression was suppressed by Ras ASODN. Sensitivity of Pc-2 cells to chemotherapy was determined by the MTT assay. MDR-1 mRNA level was detected by fluorogenic probe quantitative reverse transcription polymerase chain (RT-PCR) method. Flow cytometry (FCM) was used to detect the accumulative concentration of adriamycin (ADR) in the cells.
RESULTSRas ASODN significantly inhibited the Ras and P-gp expression (P < 0.05), increased the sensitivity of Pc-2 cells to chemotherapeutic agents (P < 0.05), decreased MDR-1 gene level in Pc-2 cells (P < 0.05), and increased the intracellular intake of ADR in Pc-2 cells (P < 0.05).
CONCLUSIONRas ASODN may enhance the sensitivity of multidrug-resistant pancreatic cancer Pc-2 cells to chemotherapeutic agents by regulating MDR-1 gene level.
