In vitro differentiation of human bone marrow-derived mesenchymal stem cells into blood vessel endothelial cells.

Feng Liang; Yun-fang Wang; Xue Nan; Hui-min Yue; Ying-xin XU; Shuang-shuang Shi; Rong LI; Xue-tao Pei

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In vitro differentiation of human bone marrow-derived mesenchymal stem cells into blood vessel endothelial cells.

Author: Feng LIANG ¹ ; Yun-fang WANG ; Xue NAN ; Hui-min YUE ; Ying-xin XU ; Shuang-shuang SHI ; Rong LI ; Xue-tao PEI
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1. Department of Stem Cell and Regenerative Medicine, Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China.
Publication Type:Journal Article
MeSH: Cell Culture Techniques; methods; Cell Differentiation; drug effects; Endothelial Cells; cytology; drug effects; Fibrin; Fibroblast Growth Factor 2; pharmacology; Gels; Humans; Mesenchymal Stromal Cells; cytology; drug effects; Vascular Endothelial Growth Factor A; pharmacology; Vascular Endothelial Growth Factor Receptor-1; metabolism; Vascular Endothelial Growth Factor Receptor-2; metabolism; von Willebrand Factor; metabolism
From: Acta Academiae Medicinae Sinicae 2005;27(6):665-669
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study differentiation of human bone marrow-derived mesenchymal stem cells (BDMSC) into blood vessel endothelial cells for ideal cell origin of complex organ tissue engineering vascularization and injured tissue repairing by cell transplantation.
METHODAfter different days of induction with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in 3D fibrin-gels and matrigel, BDMSC and angiogenesis were determined by the utilization of morphological observation, tissue section and CD34, CD31, vascular endothelial growth factor receptor-1 (VEGFR-1, Flt-1), VEGFR-2 (Flk-1), and vWF that were special for blood vessel endothelial cells.
RESULTAfter 3D-cultured and induced with VEGF and bFGF in vitro in fibrin-gels and matrigel for 3-21 days, BDMSC expressed CD34, CD31, Flt-1, Flk-1, and vWF came into vessel-like configuration.
CONCLUSIONVEGF, bFGF as well as Flt-1 and Flk-1, expressed by BDMSC, may form a feasible microenviroment after induction and play an important role during processes of blood vessel endothelial cell differentiation and vessel-like configuration forming of BDMSC. Mesenchymal stem cells may be applied to tissue engineering vascularization and injured tissue repairing by cell transplantation.