Flow cytometry analysis and differentiation study of selected nestin positive cells.
- Author:
Hong WANG
1
;
Jiang HU
;
Ling-song LI
;
Tian-pei HONG
;
Li-ying LI
Author Information
- Publication Type:Journal Article
- MeSH: Adipocytes; cytology; Bone Marrow Cells; cytology; Cell Differentiation; Cell Separation; methods; Cells, Cultured; Fetal Stem Cells; chemistry; cytology; metabolism; Flow Cytometry; Humans; Intermediate Filament Proteins; Mesenchymal Stromal Cells; metabolism; Nerve Tissue Proteins; Nestin; Pancreas; cytology; embryology
- From: Acta Academiae Medicinae Sinicae 2005;27(6):683-687
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo verify the hypothesis that selected nestin positive cells derived from human fetal pancreas (according as medical ethnics) have surface markers similar to bone marrow mesenchymal stem cells (MSCs), and that these cells have multilineage potential.
METHODThe cell surface markers were determined by flow cytometry, and then the potential that these cells might be differentiated into adipocytes and osteoplasts were explored.
RESULTThese cells have similar surface markers as MSCs of bone marrow origin. These cells was induced to differentiate into adipocytes and osteoplasts.
CONCLUSIONSelected nestin positive cells derived from human fetal pancreas have certain characteristics of MSCs.
