Chemical coupling of anti-dNA antibody on collagen coating.
- Author:
Lan-xia LIU
1
;
Xi-gang LENG
;
Jing-yin YUE
;
Li-ping SONG
;
Hai WANG
;
Cun-xian SONG
Author Information
- Publication Type:Journal Article
- MeSH: Angioplasty, Balloon, Coronary; instrumentation; Animals; Antibodies, Antinuclear; metabolism; Cattle; Coated Materials, Biocompatible; chemistry; metabolism; Collagen; chemistry; metabolism; Genetic Vectors; Immunoglobulin M; metabolism; In Vitro Techniques; Mice; Protein Binding; Stents; Surface Properties
- From: Acta Academiae Medicinae Sinicae 2005;27(6):718-722
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the feasibility and stability of chemically conjugating IgM on collagen films.
METHODSIgM was labeled with 125I using the chloramine-T method. Six collagen films were randomly divided into two groups. In chemical coupling group 125I-labeled IgM was chemically coupled with the films through N-succinmiclyl-3- (2-pyridyl-dithio) propionate reaction. In control group 125I-labeled IgM was absorbed onto collagen films. The amount of IgM on the collagen films and the amount of IgM remained on the films after extensive rinsing with phosphate buffered saline were monitored by counting the radioactivity of 125I.
RESULTSThe amount of antibodies loaded onto collagen films in the chemical coupling group was 15 times higher than that on the control films, showing significant statistical difference (P < 0.01). And the stability of conjugation antibodies on collagen films was significantly better than the control films.
CONCLUSIONChemical coupling is an effective approach to immobilize antibodies on collagen for further plasmid DNA tethering.
