Effect of testosterone propionate on the distribution pattern of calcitonin gene-related peptide in different motoneuron pools.
- Author:
Ge-ming SHI
1
;
Shuang-cheng LI
;
Kai ZHANG
;
Ling-xiao XING
;
Hui-xian CUI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcitonin Gene-Related Peptide; drug effects; metabolism; Male; Motor Neurons; drug effects; metabolism; Muscle Fibers, Fast-Twitch; cytology; drug effects; Muscle Fibers, Slow-Twitch; cytology; drug effects; Rats; Rats, Wistar; Testosterone Propionate; pharmacology
- From: Acta Academiae Medicinae Sinicae 2005;27(6):739-742
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of testosterone propionate (TP) on the distribution pattern of calcitonin gene-related peptide (CGRP) in two types of motoneuron (Mn) pools in rats.
METHODThe double labeling of cholera toxin B subunit coupled with colloidal gold (CB-Au) retrograde identification combining with immunocytochemistry was mainly used to reveal the distribution pattern of CGRP-like immunoreactivity (CGRP-LI) and its changes in the motoneuron pools labeled by CB-Au.
RESULTTP injected intramuscularly 28 days later significantly decreased CGRP expression in Mn pool innervating extensor digitorum longus (EDL, fast-twitch), comparing with corresponding control and castration group respectively (P < 0.001), while no significant effect on Mn pools innervating soleus (SOL, slow-twitch, P > 0.05) was observed.
CONCLUSIONEDL-Mn pool is more sensitive to testosterone propionate than SOL-Mn pool in regulating CGRP expression.
