DNA sequencing of miR-17-92 cluster at chromosome 13q31-q32 in mantel cell lymphoma cell lines.
- Author:
Wei XU
1
;
Jian-Yong LI
;
Qiu-Dan SHEN
;
Li LI
;
Hui YU
Author Information
1. Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Cell Line, Tumor;
Chromosomes, Human, Pair 13;
genetics;
Humans;
Lymphoma, Mantle-Cell;
genetics;
immunology;
MicroRNAs;
genetics;
Molecular Sequence Data;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2007;15(5):986-988
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the characteristics of miR-17-92 cluster at chromosome 13q31-q32 in B cell malignant lymphoma and to investigate the changes of miR-17-92 cluster in B cell lymphoma at genome DNA level and its influence on expression of miR-17-92 cluster and relation with lymphoma occurrence. PCR and DNA sequencing were used to detect miR-17-92 cluster at chromosome 13q31-q32 in mantel cell lymphoma (MCL) cell lines Rec1, G519 and Z138. The results showed that DNA sequence of miR-17-92 cluster at chromosome 13q31-q32 in MCL cell lines were normal. It is concluded that there is no abnormal change in DNA sequence of miR-17-92 cluster which is not the mechanism for miR-17-92 cluster overexpression in mantel cell lymphoma cell lines.
