Isolation and identification of mesenchymal stem cells from perfusion of human umbilical cord vein.
- Author:
Xiao-Dan LIU
1
;
Bing LIU
;
Xiu-Sen LI
;
Ning MAO
Author Information
1. Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Cell Separation;
methods;
Cells, Cultured;
Female;
Fetal Blood;
cytology;
Humans;
Mesenchymal Stromal Cells;
cytology;
Umbilical Veins;
cytology
- From:
Journal of Experimental Hematology
2007;15(5):1019-1022
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate whether mesenchymal stem cells (MSCs) existed in human umbilical cord vein and to establish the methods of isolation and expansion in vitro. The MSCs derived for perfusion of umbilical cord vein (UVMSCs) were collected after parturition of Healthy pregnant women. The morphology of MSCs and their differentiation potential into osteoblast, adipocyte and chondrocyte were observed the phenotype and cell cycle of MSCs were determined by using flow cytometry. The result showed that the mesenchymal stem cells separated from umbilical cord vein gave rise to a population of adherent cells with a typical fibroblast-like morphology. Similarly to bone marrow-derived MSCs, they highly expressed CD29, HLA-ABC, CD166, CD105, CD73 and CD44, and were negative for any hematopoietic and endothelial markers (CD45, CD34, CD14 and CD144). Functionally, they could differentiate into the osteoblast, adipocyte and chondrocyte. It is concluded that MSCs exist in the human umbilical cord vein perfusion. Their read amplification in vitro contribute to clinical applications for cell therapy and tissue engineering.
