Prokaryotic expression of HLA-B * 2705 heavy chain and identification for its activity.
- Author:
Yan-Bo LI
1
;
Yu-Ying SUN
;
Si-Qi GUO
;
Ye-Ping SUN
;
Xiu-Yun ZHANG
;
Fan-Hua KONG
;
Yong-Zhi XI
Author Information
1. Department of Immunology, Affiliated Hospital of Academy of Military Medical Sciences, Laboratory of Immunoassy, National Center of Biomedical Analysis, Beijing 100039, China.
- Publication Type:Journal Article
- MeSH:
Escherichia coli;
genetics;
metabolism;
Genetic Vectors;
genetics;
HLA-B27 Antigen;
classification;
genetics;
immunology;
metabolism;
Humans;
Immunoglobulin Heavy Chains;
genetics;
immunology;
metabolism
- From:
Journal of Experimental Hematology
2007;15(5):1028-1031
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the expression of heavy chain of HLA-B * 2705 in prokaryotic system and identify its activity, the extra-membrane gene fragment of HLA-B * 2705 was amplified from full-length HLA-B*2705 cDNA by PCR and cloned into pGEM-T vector. After identification by sequencing, the prokaryotic expressing vector pET32a (+)-B * 2705 was constructed. The antigenic activity of expressed protein was identified by Western blot and antibody blocking reaction. The results indicated that the fused HLA-B * 2705 protein expression with high efficiency was obtained. The expressed product was more than 50% of the total bacteria protein. The antigenic activity of expressed protein was confirmed by Western blot and antibody blocking reaction. It is concluded that HLA-B * 2705 fusion protein are obtained as basis for the further studies.
