Expressions of c-mpl proteins on CD34+ bone marrow cells and platelets of the patients with polycythemia vera.
- Author:
Jie BAI
1
;
Zong-Hong SHAO
;
Jun SHI
;
Hai-Rong JIA
;
Juan SUN
Author Information
1. Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences Peking Union Medical College, 300020 Tianjin, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
analysis;
Blood Platelets;
metabolism;
Bone Marrow Cells;
metabolism;
pathology;
Hematopoietic Stem Cells;
metabolism;
pathology;
Humans;
Polycythemia Vera;
genetics;
metabolism;
RNA, Messenger;
metabolism;
Receptors, Thrombopoietin;
metabolism
- From:
Journal of Experimental Hematology
2007;15(5):1061-1064
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to investigate the expressions of TPO receptor (c-mpl) proteins on CD34 positive bone marrow cells (CD34+ BMCs), platelets and the expression of c-mpl mRNA in bone marrow cells of the patients with polycythemia vera (PV). The expressions of c-mpl proteins on CD34+ bone marrow hematopoietic cells of 13 PV patients and 15 normal controls were assessed by bicolor flow cytometry and the expressions of c-mpl proteins on platelets of 15 PV patients and 15 normal controls were assessed by monocolor flow cytometry, and the expressions of c-mpl mRNA in bone marrow hematopoietic cells (BMHCs) were assessed by RT-PCR. The results showed that no difference was found between the expression of c-mpl proteins on CD34+ BMHCs of PV patients (0.99% +/- 0.14%) and that of normal controls (0.92% +/- 0.12%) (p > 0.05). There was no difference too between the expression of c-mpl protein on platelets in PV patients (20.33% +/- 4.84%) and that in normal controls (23.50% +/- 3.64%) (p > 0.05). No difference between the expression of c-mpl mRNA in BMHCs of PV patients and that in normal controls was seen. In conclusion, the expressions of c-mpl proteins on CD34+ BMHCs, platelets and c-mpl mRNA in BMHCs of PV patients were not obviously abnormal.
