Effect of liposomal transfection of antisense oligodeoxynucleotide on alpha-globin gene expression and proliferation of K562 cells.
- Author:
Rong-Rong LIU
1
;
Yong-Rong LAI
;
Jie MA
Author Information
1. Department of Hematology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
drug effects;
Gene Expression;
Humans;
K562 Cells;
Liposomes;
metabolism;
Oligonucleotides, Antisense;
pharmacology;
Transfection;
alpha-Globins;
metabolism
- From:
Journal of Experimental Hematology
2007;15(5):1065-1069
- CountryChina
- Language:Chinese
-
Abstract:
The objective of study was to investigate the effect of liposomal transfection of antisense oligodeoxynucleotide (ASON) on alpha-globin gene expression and proliferation of K562 cells, to explore the new way of gene therapy in beta-thalassemia. Targeted ASON of alpha-globin was designed and synthesized, and compared with positive control [sense oligodeoxynucleotide (SON) group] and blank control. By liposomal transfection, ASON, SON was co-cultured with K562. The efficiency of transfection was assayed by fluorescence microscopy and flow cytometry (FCM), the alpha-globin gene expression of K562 was measured by real-time PCR, and the proliferation of K562 was determined by Cell Count Kit-8 assay. The results indicated that the highest efficiency was at 24 hours after liposomal transfection, the gene expression level of alpha-globin in ASON group was significantly lower than that in SON group and blank control (p < 0.01). The proliferation of K562 cells was obviously inhibited, meanwhile the above effect showed the dose-dependent manner. It is concluded that the liposomal transfection of ASON inhibits the alpha-globin gene expression of K562 cells, which may be the new target for gene therapy in beta-thalassemia.
