Sequence analysis of a novel HLA-A * 2459 allele.
- Author:
Wei ZHANG
1
;
Jun-Jun HE
;
Qin-Feng LÜ
;
Wei WANG
;
Zhe-Dong HANG
;
Fa-Ming ZHU
;
Li-Xing YAN
Author Information
1. Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian Continental Ancestry Group;
genetics;
Base Sequence;
Blood Donors;
China;
HLA-A Antigens;
genetics;
Humans;
Molecular Sequence Data;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2007;15(5):1090-1092
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the molecular genetics basis of a novel allele HLA-A * 2459 in Chinese population. DNA was extracted from whole blood by PEL-FREEZ DNA extraction kit. The amplification of HLA-A exons 2 - 4 of the proband was preformed by allele specific primer PCR and the amplified product was sequenced bidirectionally with primers. The sequencing results showed HLA-A alleles of the proband as A * 1101 and the novel allele. The sequences of the novel allele have been submitted to GenBank (DQ313255, DQ313256, DQ313257). After Blast HLA analysis, the novel allele showed only one nucleotide differences with HLA-A * 24020101 at nucleotide position 527 T to C in exon 3. This results in an amino acid changes from Val to Ala at codon 152. In conclusion, this allele is a novel one and has been officially named HLA-A * 2459 by the WHO Nomenclature Committee.
