Effects of As2O3, dexamethasone and thalidomide on apoptosis and cytoplasmic Ca2+ of myeloma cell line U266.
- Author:
Ru-Feng LIN
1
;
Hua LU
;
Peng LIU
;
Yong-Ren WANG
;
Wen-Yi SHEN
;
Yu-Jie WU
;
Jian-Fu ZHANG
;
Xiao-Ming FEI
;
Zheng GE
;
Jian-Yong LI
Author Information
1. Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Arsenicals;
pharmacology;
Calcium;
metabolism;
Cell Line, Tumor;
Cytoplasm;
metabolism;
Dexamethasone;
pharmacology;
Humans;
Multiple Myeloma;
pathology;
Oxides;
pharmacology;
Thalidomide;
pharmacology
- From:
Journal of Experimental Hematology
2007;15(6):1200-1203
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the influence of As2O3, dexamethasone (Dex) and thalidomide (Thal) on apoptosis-induced myeloma cell line U266 cytoplasmic calcium concentrations ([Ca2+]i), U266 cells were incubated in the culture of RPMI 1640 with 15% FBS in 24-well plate and exposed to different concentrations of As2O3, Dex and Thal for 8 hours, respectively, then cell apoptosis was analyzed by fluorescence microscopy and flow cytometry (FCM) with Annexin V-FITC/PI double staining, and cytoplasmic free calcium were detected on FCM through Fluo-3/AM loading. The results indicated that (1) apoptotic cells were gradually increased with enhancement of As2O3, Dex and Thal concentrations; (2) apoptotic cell rates increased from 0.56% in control to 31.54%, 28.35% and 21.97% respectively after treatment with As2O3, Dex and Thal; (3) As2O3, Dex induced U266 cell apoptosis accompanied with raise of [Ca2+]i; (4) [Ca2+]i had no statistically significant changes in Thal-induced apoptotic U266 cells. It is concluded that the raise of [Ca2+]i is one of the mechanisms for As2O3 and Dex-induced U266 cells apoptosis, whereas Thal-induced U266 apoptosis has no significant relation to [Ca2+]i changes.
