Effect of human bone marrow mesenchymal stem cells on T lymphocyte killing K562 cells.
- Author:
Zhi-Gang SI
1
;
Hai BAI
;
Cun-Bang WANG
;
Zhi-Wen XUE
;
Qian WANG
;
Tao WU
;
Xiao-Liang YANG
Author Information
1. Department of Hematology, General Hospital of PLA Lanzhou Military Area, Lanzhou 730050, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
cytology;
CD4 Antigens;
immunology;
Coculture Techniques;
Humans;
Interferon-gamma;
metabolism;
Interleukin-2 Receptor alpha Subunit;
immunology;
Interleukin-4;
metabolism;
K562 Cells;
Mesenchymal Stromal Cells;
cytology;
immunology;
T-Lymphocyte Subsets;
immunology;
metabolism;
T-Lymphocytes;
cytology;
immunology
- From:
Journal of Experimental Hematology
2007;15(6):1216-1219
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to investigate the effect of human bone marrow mesenchymal stem cells (MSCs) on T lymphocyte killing K562 cells. MSCs were isolated from bone marrow and cultured, T cells were harvested by using nylon column method from peripheral blood. The T cells were co-cultured with MSCs, the phenotype expressions of T cell subsets were detected by flow cytometry. Killing effects of T cells (culture alone and co-culture with MSCs) on K562 cells were detected by LDH, expressions of IFN-gamma and IL-4 were detected by ELISA. The results showed that after T cells were co-cultured with MSCs for three days, the proportion of CD4+ and CD4+CD25+ T cells raised significantly (p<0.05) as compared with group of culture alone, but the proportion of CD8+ T cell were not significantly changed (p>0.05). In group of T cells co-cultured with MSCs, killing effects of T cells on K562 cells weakened, at the same time, expression of IFN-gamma decreased while expression of IL-4 increased. It is concluded that the MSCs weaken killing effects of T cells on K562 cells, which associates with increase of CD4+CD25+ T cell subsets and changes of IFN-gamma and IL-4 levels.
