Effect of tetrandrine, toremifene and their combination on the reversion of multidrug resistance of K562/A02 cell line.
- Author:
Qiu-Xia ZHAO
1
;
Bao-An CHEN
;
Jian CHENG
;
Jia-Hua DING
;
Feng GAO
;
Chong GAO
;
Yun-Yu SUN
;
Jun WANG
;
Gang ZHAO
;
Wen BAO
;
Hui-Hui SONG
Author Information
1. Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical College, Nanjing 210009, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Hormonal;
pharmacology;
Antineoplastic Agents, Phytogenic;
pharmacology;
Benzylisoquinolines;
pharmacology;
Doxorubicin;
Drug Resistance, Multiple;
drug effects;
Drug Resistance, Neoplasm;
drug effects;
Drug Synergism;
Humans;
K562 Cells;
Toremifene;
pharmacology
- From:
Journal of Experimental Hematology
2008;16(1):61-64
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the reversible effect of tetrandrine, toremifene and their combination on multidrug resistance of K562/A02 cell line. The IC(50) (the concentration causing 50% inhibition of cell growth) of adriamycin (ADR) were assayed by MTT method, the expression of MDR1 mRNA was measured by RT-PCR, the concentration of p-glycoprotein (P-gp) and intracellular ADR were detected by flow cytometry. The results showed that the IC(50) of ADR on K562/A02 and K562 cells were 57.43 and 1.16 mg/L, respectively. The IC(50) of ADR on K562/A02 cells after treatment with tetrandrine, toremifene and both combination were 14.12, 20.74 and 9.14 mg/L respectively, but both drugs did not influence the IC(50) of ADR on K562 cells. Pretreating K562/A02 cells with toremifene (2.5 micromol/L), tetrandrine (1 micromol/L) or both for 72 hours partially restored the sensitivity of K562/A02 cells to ADR. Tetrandrine and toremifene (alone or combination) elevated the ADR concentration in K562/A02, down regulated the expressions of P-gp and MDR1 mRNA. It is concluded that multidrug resistance of K562/A02 cells can be partially reversed by tetrandrine or toremifene, the combination of both drugs shows a higher synergistic reversal effect.
