Influence of tetrandrine on SORCIN gene expression in K562/A02 cell line.
- Author:
Jing LI
1
;
Bao-An CHEN
;
Min-Sheng ZHU
;
Fen GAO
;
Jia-Hua DING
;
Chong GAO
;
Yun-Yu SUN
;
Jian CHENG
;
Jun WANG
;
Gang ZHAO
;
Hui-Hui SONG
;
Yan MA
;
Wen BAO
;
Xin-Cheng SUN
;
Hong-Yan CHENG
;
Wen-Lin XU
;
Hui-Ling SHEN
Author Information
1. Department of Hematology, Zhongda Hospital, Southeast University, Nanjing 210009, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
pharmacology;
Benzylisoquinolines;
pharmacology;
Calcium-Binding Proteins;
genetics;
metabolism;
Doxorubicin;
Drug Resistance, Multiple;
drug effects;
Drug Resistance, Neoplasm;
drug effects;
Humans;
K562 Cells
- From:
Journal of Experimental Hematology
2008;16(1):65-69
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the changes of soluble resistance-related calcium binding protein (sorcin) expression in reversion of multidrug resistance of K562/A02 leukemic cell line with different concentrations of tetrandrine (Tet), so as to provide a new theoretic evidence for clinical application of Tet. The inhibition of K562/A02 cell line by daunorubicin (DNR) was assayed by MTT method. The changes of SORCIN gene expression were assayed by RT-PCR. The changes of SORCIN protein expressed were assayed by Western blot. The results showed that Tet could enhance the cytotoxicity of DNR to K562/A02 cells (the IC(50) of DNR + Tet was 11.3+/-0.17 mg/L, 5.15+/-0.10 mg/L, 3.91+/-0.06 mg/L, and 2.52+/-0.04 mg/L, when concentrations of Tet were 0 mg/L, 0.5 mg/L, 1.0 mg/L, and 2.0 mg/L respectively). The gene encoding sorcin was highly expressed in K562/A02 cells, the expression of which was firstly enhanced in Tet concentration 0.5 mg/L, then attenuated in Tet concentration of 1.0, 2.0 mg/L (p<0.05). Sorcin protein expressed lowly in K562 cells and highly in K562/A02 cells, but the expression of SORCIN protein in K562/A02 cells was enhanced in Tet concentration of 0.5 mg/L, then was attenuated in Tet concentration of 1.0, 2.0 mg/L (p<0.05). It is concluded that the effect of Tet on reversal of K562/A02 drug-resistance shows concentration dependence by MTT assay. Tet reverses multidrug-resistance of K562/A02 cells through regulation of expression of SORCIN gene and protein, but not fully correlates to the reversing effect.
