Determination of plasma protein binding rate of arctiin and arctigenin with ultrafiltration.
- Author:
Xue-Ying HAN
1
;
Wei WANG
;
Ri-Qiu TAN
;
De-Qiang DOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Binding, Competitive; Blood Proteins; metabolism; Chromatography, High Pressure Liquid; Furans; metabolism; Glucosides; metabolism; Humans; Lignans; metabolism; Male; Protein Binding; Rats; Rats, Sprague-Dawley; Ultrafiltration; methods
- From: China Journal of Chinese Materia Medica 2013;38(3):432-434
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the plasma protein binding rate of arctiin and arctigenin.
METHODThe ultrafiltration combined with HPLC was employed to determine the plasma protein binding rate of arctiin and arctigenin as well as rat plasma and healthy human plasma proteins.
RESULTThe plasma protein binding rate of arctiin with rat plasma at the concentrations of 64. 29, 32.14, 16.07 mg x L(-1) were (71.2 +/- 2.0)%, (73.4 +/- 0.61)%, (78.2 +/- 1.9)%, respectively; while the plasma protein binding rate of arctiin with healthy human plasma at the above concentrations were (64.8 +/- 3.1)%, (64.5 +/- 2.5)%, (77.5 +/- 1.7)%, respectively. The plasma protein binding rate of arctigenin with rat plasma at the concentrations of 77.42, 38.71, 19.36 mg x L(-1) were (96.7 +/- 0.41)%, (96.8 +/- 1.6)%, (97.3 +/- 0.46)%, respectively; while the plasma protein binding rate of arctigenin with normal human plasma at the above concentrations were (94.7 +/- 3.1)%, (96.8 +/- 1.6)%, (97.9 +/- 1.3)%, respectively.
CONCLUSIONThe binding rate of arctiin with rat plasma protein was moderate, which is slightly higher than the binding rate of arctiin with healthy human plasma protein. The plasma protein binding rates of arctigenin with both rat plasma and healthy human plasma are very high.
