Principles for molecular identification of traditional Chinese materia medica using DNA barcoding.
- Author:
Shi-Lin CHEN
1
;
Hui YAO
;
Jian-Ping HAN
;
Tian-Yi XIN
;
Xiao-Hui PANG
;
Lin-Chun SHI
;
Kun LUO
;
Jing-Yuan SONG
;
Dian-Yun HOU
;
Shang-Mei SHI
;
Zhong-Zhi QIAN
Author Information
1. The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. slchen@implad.ac.cn
- Publication Type:Journal Article
- MeSH:
Animals;
China;
DNA;
genetics;
DNA Barcoding, Taxonomic;
methods;
DNA, Ribosomal Spacer;
genetics;
Drugs, Chinese Herbal;
classification;
isolation & purification;
Electron Transport Complex IV;
genetics;
Materia Medica;
classification;
isolation & purification;
Medicine, Chinese Traditional;
Plant Proteins;
genetics;
Plants, Medicinal
- From:
China Journal of Chinese Materia Medica
2013;38(2):141-148
- CountryChina
- Language:Chinese
-
Abstract:
Since the research of molecular identification of Chinese Materia Medica (CMM) using DNA barcode is rapidly developing and popularizing, the principle of this method is approved to be listed in the Supplement of the Pharmacopoeia of the People's Republic of China. Based on the study on comprehensive samples, the DNA barcoding systems have been established to identify CMM, i.e. ITS2 as a core barcode and psbA-trnH as a complementary locus for identification of planta medica, and COI as a core barcode and ITS2 as a complementary locus for identification of animal medica. This article introduced the principle of molecular identification of CMM using DNA barcoding and its drafting instructions. Furthermore, its application perspective was discussed.
