Simultaneous determination of four flavones in root and stem of Cudrania tricuspidata and C. cochinchinensis by HPLC-DAD.
- Author:
Bo LI
1
;
Mei WANG
;
Ya-Nan TAN
;
Miao-Miao TONG
;
Yan-Jun ZHAI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Flavanones; chemistry; isolation & purification; Flavones; chemistry; isolation & purification; Kaempferols; chemistry; isolation & purification; Methanol; Moraceae; chemistry; Organ Specificity; Phosphoric Acids; Plant Roots; chemistry; Plant Stems; chemistry; Plants, Medicinal; Quercetin; analogs & derivatives; chemistry; isolation & purification; Reproducibility of Results; Species Specificity
- From: China Journal of Chinese Materia Medica 2013;38(2):167-170
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC-DAD method for the determination of axifolin, naringenin, quercetin and kaempferol in Cudrania tricuspidata and C. cochinchinensis in order to provide a scientific reference for species identification and quality evaluation, by establishing.
METHODThe determination was performed by HPLC-DAD on an Agilent C18 column (4.6 mm x 150 mm, 5 microm) by gradient elution (0-15 min, 35%-50% A; 15-30 min, 50% - 65% A) using methanol (A) and 0.1% phosphoric acid (B) as the mobile phase. The flow rate was 1 mL x min(-1). The detection wavelength was 290 nm for taxifolin and naringenin, 365 nm for quercetin and kaempferol with column temperature at 30 degrees C.
RESULTThe content of axifolin and quercetin in the root of C. tricuspidata were remarkably higher than that in the root of C. cochinchinensis, and the content in stem of C. tricuspidata was also higher than that in the stem of C. cochinchinensis, the content of axifolin and quercetin was variable in different species. The content of naringenin and kaempferol in the root of C. cochinchinensis was visibly higher than that in the root of C. tricuspidata, and the content in the stems of the two herbs was similar, the content of naringenin and kaempferol was visibly variable in different medicinal parts of the herb, but similar between the two herbs.
CONCLUSIONThere's some difference of the content of the four ingredients in different medicinal parts and different herbs, so clinical use should not be confused.
