Change in dissolution of chemical components of frankincense-myrrh before and after their compatibility and effect on no release of LPS-induced macrophage cells.
- Author:
Ting CHEN
1
;
Shu-Lan SU
;
Jin-Ao DUAN
;
Er-Xin SHANG
;
Da-Wei QIAN
;
Yu-Ping TANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents, Non-Steroidal; chemistry; isolation & purification; Boswellia; chemistry; Chromatography, Liquid; Commiphora; chemistry; Drug Synergism; Drugs, Chinese Herbal; chemistry; isolation & purification; Lipopolysaccharides; pharmacology; Macrophages, Peritoneal; drug effects; Male; Mice; Mice, Inbred BALB C; Molecular Structure; Nitric Oxide; metabolism; Tandem Mass Spectrometry; Terpenes; chemistry; isolation & purification; Triterpenes; chemistry; isolation & purification
- From: China Journal of Chinese Materia Medica 2013;38(2):179-185
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the difference of chemical compounds of frankincense-myrrh before and after their compatibility, and evaluate the effect of differentiated compounds on NO generated by LPS-induced peritoneal macrophage cells in rats, in order to discuss synergetic material basis of frankincense-myrrh compatibility from the prospective of change in chemical constituents.
METHODUPLC-Q-TOF-MS/MS combined technology was used to analyze the chemical components of frankincense-myrrh before and after their compatibility. MarkerLynx 4. 1 statistical software was used to analyze differentiated compounds before and after their compatibility.
RESULTThe results of PCA showed that there were significant differences in the combined extracts of frankincense-myrrh and the chromatogram of their combined liquid, suggesting significant differences in their chemical compounds before and after their compatibility; after their compatibility, the dissolution of pentacyclic triterpenoid (alpha-boswellic acid, beta-boswellic acid) and tetracyclic triterpenoid (elemonic acid, 3-acetoxy-16-hydroxy-dammar-24-ene, 3-hydroxytirucalla-8,24-dien-21-oic acid or 3-hydroxytirucalla-7,24-dien-21-oic acid) increased notably, while the dissolution of both yclic sesquiterpenes and macrocyclic diterpenoids decreased. According to the evaluation on in vitro activity, 2-methoxy-8, 12-epoxy-germa-1 (10), 7, 11-triene-6-ketone, 2-methoxy-5-acetoxyl-furan-germa-1 (10)-alkene-6-ketone and 3-carbonyl Euphorbia kansui-8, 24-diene-21-carboxylic acid notably inhibited NO generated by LPS-induced peritoneal macrophage cells in rats.
CONCLUSIONThese findings provide scientific basis and reference for studies on anti-inflammatory material basis of frankincense-myrrh compatibility.
