Rapid recognition and identification of chemical constituents in Qinghuo Zhimai tablets by SPE-HPLC-ESI-MS(n).
- Author:
Jia-Yu ZHANG
1
;
Yan-Jiang QIAO
;
Xiao-Yan GAO
;
Qian ZHANG
;
Qun MA
;
Jian-Qiu LU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Flavones; chemistry; isolation & purification; Glycosides; chemistry; isolation & purification; Iridoids; chemistry; isolation & purification; Lactones; chemistry; isolation & purification; Medicine, Chinese Traditional; Molecular Structure; Quinic Acid; analogs & derivatives; chemistry; isolation & purification; Saponins; chemistry; isolation & purification; Solid Phase Extraction; methods; Spectrometry, Mass, Electrospray Ionization; methods; Tablets; Tandem Mass Spectrometry; methods
- From: China Journal of Chinese Materia Medica 2013;38(2):186-192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for analyzing solid phase extraction-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (SPE-HPLC-ESI-MS(n)), in order to recognize and identify the main chemical constituents in Qinghuo Zhimai tablets.
METHODThe possible structures of the compounds were determined according to the structure information of compounds observed from molecular ion peaks and fragment ions in HPLC-ESI-MS(n) negative ion mode and by comparing with literature data or control samples.
RESULTThrough the comparative analysis on Qinghuo Zhimai Tablets and components of its formula, 39 chemical constituents were identified, including 7 caffeoylquinic acids, 7 iridoids, 6 diterpenoid lactones, 5 homoisoflavonoids, 13 steroidal saponins and 1 flavone glycoside.
CONCLUSIONThis study provides a simple and rapid method for identifying chemical components in Qinghuo Zhimai tablets.
